With targeted resequencing, a subset of genes or regions of the genome are isolated and sequenced. Targeted approaches using next generation sequencing (NGS) allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome, specific genes of interest (custom content), targets within genes, or mitochondrial DNA.
Affordable Sequencing With Focused Content
Compared to broader approaches, such as whole-genome sequencing, targeted resequencing is a more cost-effective method for investigating areas of interest. Targeted resequencing enables researchers to focus on regions that are most likely to be involved in the phenotype under study, conserving resources and generating a smaller, more manageable data set. Targeted approaches can also deliver much higher coverage levels, allowing identification of variants that would be too rare and too expensive to identify with whole-genome or Sanger sequencing.
Advantages:
- Focuses on regions of interest, generating a smaller, more manageable data set
- Reduces sequencing costs and data analysis burdens
- Reduces turnaround time compared to broader approaches
- Enables deep sequencing at high coverage levels for rare variant identification