{"id":13,"date":"2015-12-07T06:39:33","date_gmt":"2015-12-07T06:39:33","guid":{"rendered":"http:\/\/ilyabiotech.com\/eng\/?page_id=13"},"modified":"2024-08-21T14:05:44","modified_gmt":"2024-08-21T14:05:44","slug":"ilya-taq-master-mix","status":"publish","type":"page","link":"http:\/\/ilyabiotech.com\/eng\/products\/ilya-taq-master-mix\/","title":{"rendered":"Ilya Taq Master Mix"},"content":{"rendered":"

Ilya Taq DNA Polymerase Master Mix RED 2X<\/strong><\/p>\n

1.5 mM MgCl2 final concentration <\/strong><\/p>\n

Key Features and General Description<\/strong> \"mastermix<\/a><\/p>\n

\u2013\u00a0High performance thermostable Ilya Taq DNA polymerase<\/p>\n

\u2013\u00a0Significantly reduced set up time<\/p>\n

\u2013\u00a0Direct loading of products onto agarose gels<\/p>\n

\u2013\u00a0Easy visualization of complete mixing<\/p>\n

\u2013\u00a0Increased reproducibility<\/p>\n

\u2013\u00a0Diminished risk of contamination<\/p>\n

\u2013\u00a0No proofreading \u2013 lacks a 3\u2032-5\u2032 exonuclease activity<\/p>\n

\u2013\u00a0Ideal for TA cloning \u2013 leaves an A overhang<\/p>\n

Taq DNA Polymerase 2x Master Mix RED is a ready-to-use 2x reaction mix with the Ilya Taq DNA polymerase which is engineered by Ilya Biotech Co. Ltd. This master mix includes the NH4+ buffer system, dNTPs and magnesium chloride. Simply add primers, template and water to a total reaction volume of 50 \u03bcl to successfully carry out primer extensions and other molecular biology applications.<\/p>\n

There is no need to buy and use separate loading dyes. Simply load a portion of the reaction product onto an agarose gel for electrophoresis and subsequent visualization.<\/p>\n

Composition of the Taq DNA Polymerase 2x Master Mix RED (1.5 mM MgCl2 final concentration) <\/strong><\/p>\n

\u2013\u00a0Tris-HCl pH 8.5, (NH4)2S04, 3 mM MgCl2, 0.2% Tween\u00d2 20<\/p>\n

\u2013\u00a00.4 mM of each dNTP<\/p>\n

\u2013\u00a00.2 units\/\u03bcl Ilya Taq DNA polymerase<\/p>\n

\u2013\u00a0Red dye (inert)<\/p>\n

\u2013\u00a0stabilizer<\/p>\n

Storage and Stability <\/strong><\/p>\n

Ilya Taq DNA Polymerase Master Mix RED 2X kit is stable at -20 \u00b0C for 2 years after the production date.<\/p>\n

Quality Control <\/strong><\/p>\n

Taq DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity, exonuclease activity or priming activity.<\/p>\n

Unit Definition <\/strong><\/p>\n

One unit is defined as the amount of Ilya Taq DNA polymerase that incorporates 10 nmoles of dNTPs into acid-precipitable DNA in 30 minutes at 72 \u00b0C under standard assay conditions.<\/p>\n

Suggested Protocol Using Ilya Taq Master Mix RED 2x<\/strong><\/p>\n

This protocol serves as a guideline for primer extensions. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be determined individually.<\/p>\n

Note:<\/strong><\/p>\n

n The final MgCl2 concentration of this Taq Master Mix RED is 1.5 mM 2x. In some applications, more than 1.5 mM MgCl2 is required for best results. Use 25 mM MgCl2 (may be purchased separately) to adjust the Mg2+ concentration according to table1:<\/p>\n\n\n\n\n
Final MgCl2 concentration in reaction (mM)<\/td>\n1.5<\/td>\n2.0<\/td>\n2.5<\/td>\n3.0<\/td>\n3.5<\/td>\n4.0<\/td>\n4.5<\/td>\n<\/tr>\n
Volume of 25 mM MgCl2<\/td>\n0<\/td>\n1<\/td>\n2<\/td>\n3<\/td>\n4<\/td>\n5<\/td>\n6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n