{"id":11,"date":"2015-12-07T06:36:29","date_gmt":"2015-12-07T06:36:29","guid":{"rendered":"http:\/\/ilyabiotech.com\/eng\/?page_id=11"},"modified":"2024-08-21T14:08:55","modified_gmt":"2024-08-21T14:08:55","slug":"products","status":"publish","type":"page","link":"http:\/\/ilyabiotech.com\/eng\/products\/","title":{"rendered":"products"},"content":{"rendered":"\n<style>\n        \/* Add your CSS styles for tabs and content here *\/\n        .tabs {\n            display: flex;\n            cursor: pointer;\n        }\n        \n        .tab {\n            padding: 10px 20px;\n            background-color: #f1f1f1;\n        }\n        \n        .active {\n            background-color: #ccc;\n        }\n        \n        .content {\n            display: none;\n            padding: 20px;\n            border: 1px solid #ccc;\n        }\n    <\/style>\n    <script src=\"https:\/\/code.jquery.com\/jquery-3.6.0.min.js\"><\/script>\n    <script>\n        $(document).ready(function() {\n            \/\/ Show the default active tab and its content\n            $(\"#tab1\").show();\n            \n            \/\/ Add active class to the default active tab\n            $(\".tab[data-target='tab1']\").addClass(\"active\");\n            \n            \/\/ Handle tab clicks\n            $(\".tab\").click(function() {\n                \/\/ Remove active class from all tabs\n                $(\".tab\").removeClass(\"active\");\n                \n                \/\/ Add active class to the clicked tab\n                $(this).addClass(\"active\");\n                \n                \/\/ Hide all content divs\n                $(\".content\").hide();\n                \n                \/\/ Show the selected content\n                var target = $(this).attr(\"data-target\");\n                $(\"#\" + target).show();\n            });\n        });\n    <\/script>\n\n    <div class=\"tabs\">\n        <div class=\"tab\" data-target=\"tab1\">Ilya GelGreen<\/div>\n        <div class=\"tab\" data-target=\"tab2\">Ilya Taq Master Mix<\/div>\n    <\/div>\n    <div id=\"tab1\" class=\"content\">\n       <div class=\"entry-content clearfix\">\n\t\t<p>GelGree is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels.&nbsp;It is far more sensitive than SYBR\u00ae Safe. Unlike SYBR\u00ae dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally. GelGreen\u2122 is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as blue LED light box, 488 nm laser-based gel scanner, or Dark Reader).<\/p>\n<p><a href=\"http:\/\/ilyabiotech.com\/eng\/wp-content\/uploads\/2015\/12\/gel-etbr.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-48 aligncenter\" src=\"http:\/\/ilyabiotech.com\/eng\/wp-content\/uploads\/2015\/12\/gel-etbr.jpg\" alt=\"gel-etbr\" width=\"221\" height=\"300\" srcset=\"http:\/\/ilyabiotech.com\/eng\/wp-content\/uploads\/2015\/12\/gel-etbr.jpg 221w, https:\/\/ilyabiotech.com\/eng\/wp-content\/uploads\/2015\/12\/gel-etbr.jpg 438w\" sizes=\"(max-width: 221px) 100vw, 221px\"><\/a><\/p>\n<p style=\"text-align: center;\"><strong>Figure<\/strong>: staining with Ethidium bromide and GelGreen in same conditions<\/p>\n<p>&nbsp;<\/p>\n<p><strong>FEATURES<\/strong><\/p>\n<ul>\n<li><strong> Safer than EB: <\/strong>Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic<\/li>\n<li><strong> Easy disposal:<\/strong> Passed environmental safety tests for direct disposal down the drain or in regular trash<\/li>\n<li><strong>Ultra-sensitive:<\/strong> Much more sensitive than EtBr and SYBR\u00ae Safe<\/li>\n<li><strong>Extremely stable: <\/strong>Available in water, stable at room temperature for long-term storage and microwavable<\/li>\n<li><strong>Simple to use: <\/strong>Very simple procedures for precast or post-electrophoresis gel staining<\/li>\n<li><strong>Compatible with a standard UV transilluminator or a gel reader with visible light excitation: <\/strong>Replaces SYBR\u00ae or GelStar\u00ae with no optical setting change<\/li>\n<li><strong> Compatible with downstream applications: <\/strong>Compatible with gel purification, restriction digest, sequencing and cloning.<\/li>\n<\/ul>\n\t\t\t<\/div>\n    <\/div>\n    <div id=\"tab2\" class=\"content\">\n       <div class=\"entry-content clearfix\">\n\t\t<p style=\"text-align: center;\"><strong>Ilya Taq DNA Polymerase Master Mix RED 2X<\/strong><\/p>\n<p><strong>1.5 mM MgCl2 final concentration <\/strong><\/p>\n<p><strong>Key Features and General Description<\/strong> <a href=\"http:\/\/ilyabiotech.com\/eng\/wp-content\/uploads\/2015\/12\/mastermix-3.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-25 alignright\" src=\"http:\/\/ilyabiotech.com\/eng\/wp-content\/uploads\/2015\/12\/mastermix-3.jpg\" alt=\"mastermix 3\" width=\"290\" height=\"239\" srcset=\"http:\/\/ilyabiotech.com\/eng\/wp-content\/uploads\/2015\/12\/mastermix-3.jpg 300w, https:\/\/ilyabiotech.com\/eng\/wp-content\/uploads\/2015\/12\/mastermix-3.jpg 600w\" sizes=\"(max-width: 290px) 100vw, 290px\"><\/a><\/p>\n<p>\u2013&nbsp;High performance thermostable Ilya Taq DNA polymerase<\/p>\n<p>\u2013&nbsp;Significantly reduced set up time<\/p>\n<p>\u2013&nbsp;Direct loading of products onto agarose gels<\/p>\n<p>\u2013&nbsp;Easy visualization of complete mixing<\/p>\n<p>\u2013&nbsp;Increased reproducibility<\/p>\n<p>\u2013&nbsp;Diminished risk of contamination<\/p>\n<p>\u2013&nbsp;No proofreading \u2013 lacks a 3\u2032-5\u2032 exonuclease activity<\/p>\n<p>\u2013&nbsp;Ideal for TA cloning \u2013 leaves an A overhang<\/p>\n<p>&nbsp;<\/p>\n<p>Taq DNA Polymerase 2x Master Mix RED is a ready-to-use 2x reaction mix with the Ilya Taq DNA polymerase which is engineered by Ilya Biotech Co. Ltd. This master mix includes the NH4+ buffer system, dNTPs and magnesium chloride. Simply add primers, template and water to a total reaction volume of 50 \u03bcl to successfully carry out primer extensions and other molecular biology applications.<\/p>\n<p>There is no need to buy and use separate loading dyes. Simply load a portion of the reaction product onto an agarose gel for electrophoresis and subsequent visualization.<\/p>\n<p><strong>Composition of the Taq DNA Polymerase 2x Master Mix RED (1.5 mM MgCl2 final concentration) <\/strong><\/p>\n<p>\u2013&nbsp;Tris-HCl pH 8.5, (NH4)2S04, 3 mM MgCl2, 0.2% Tween\u00d2 20<\/p>\n<p>\u2013&nbsp;0.4 mM of each dNTP<\/p>\n<p>\u2013&nbsp;0.2 units\/\u03bcl Ilya Taq DNA polymerase<\/p>\n<p>\u2013&nbsp;Red dye (inert)<\/p>\n<p>\u2013&nbsp;stabilizer<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Storage and Stability <\/strong><\/p>\n<p>Ilya Taq DNA Polymerase Master Mix RED 2X kit is stable at -20 \u00b0C for 2 years after the production date.<\/p>\n<p><strong>Quality Control <\/strong><\/p>\n<p>Taq DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity, exonuclease activity or priming activity.<\/p>\n<p><strong>Unit Definition <\/strong><\/p>\n<p>One unit is defined as the amount of Ilya Taq DNA polymerase that incorporates 10 nmoles of dNTPs into acid-precipitable DNA in 30 minutes at 72 \u00b0C under standard assay conditions.<\/p>\n<p><strong>Suggested Protocol Using Ilya Taq Master Mix RED 2x<\/strong><\/p>\n<p>This protocol serves as a guideline for primer extensions. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be determined individually.<\/p>\n<p><strong>Note:<\/strong><\/p>\n<p>n The final MgCl2 concentration of this Taq Master Mix RED is 1.5 mM 2x. In some applications, more than 1.5 mM MgCl2 is required for best results. Use 25 mM MgCl2 (may be purchased separately) to adjust the Mg2+ concentration according to table1:<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"247\">Final MgCl2 concentration in reaction (mM)<\/td>\n<td width=\"39\">1.5<\/td>\n<td width=\"39\">2.0<\/td>\n<td width=\"39\">2.5<\/td>\n<td width=\"39\">3.0<\/td>\n<td width=\"39\">3.5<\/td>\n<td width=\"39\">4.0<\/td>\n<td width=\"39\">4.5<\/td>\n<\/tr>\n<tr>\n<td width=\"247\">Volume of 25 mM MgCl2<\/td>\n<td width=\"39\">0<\/td>\n<td width=\"39\">1<\/td>\n<td width=\"39\">2<\/td>\n<td width=\"39\">3<\/td>\n<td width=\"39\">4<\/td>\n<td width=\"39\">5<\/td>\n<td width=\"39\">6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<ul>\n<li>Thaw Ilya Taq Master Mix RED 2X. <strong>It is important to mix thoroughly. A Homogeneous solution should be used to avoid localized concentrations of salts<\/strong>.<\/li>\n<li>Prepare a reaction mix. Table 2 shows the reaction set up for a final volume of 50 \u03bcl.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"113\">Component<\/td>\n<td width=\"79\"><strong>Vol.\/reaction<\/strong><\/td>\n<td width=\"162\"><strong>Final concentration*<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">Ilya Taq Master Mix 2X<\/td>\n<td width=\"79\">25 \u03bcl<\/td>\n<td width=\"162\">1x<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">25 mM MgCl2<\/td>\n<td width=\"79\">0 \u03bcl (0 \u2013 7 \u03bcl)<\/td>\n<td width=\"162\">1.5 mM (0.5 \u2013 5 mM)<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">Primer A (10 \u03bcM)<\/td>\n<td width=\"79\">1 \u03bcl (0.5 \u2013 5 \u03bcl)<\/td>\n<td width=\"162\">0.2 \u03bcM (0.1 \u2013 1.0 \u03bcM)<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">Primer B (10 \u03bcM)<\/td>\n<td width=\"79\">1 \u03bcl (0.5 \u2013 5 \u03bcl)<\/td>\n<td width=\"162\">0.2 \u03bcM (0.1 \u2013 1.0 \u03bcM)<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">PCR-grade H2O<\/td>\n<td width=\"79\">X \u03bcl<\/td>\n<td width=\"162\">\u2013<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">Template DNA<\/td>\n<td width=\"79\">X \u03bcl<\/td>\n<td width=\"162\">genomic DNA: 50 ng (10 \u2013 500 ng)<p><\/p>\n<p>plasmid DNA: 0.5 ng (0.1 \u2013 1 ng)<\/p>\n<p>bacterial DNA: 5 ng (1 \u2013 10 ng)<\/p><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong>TOTAL volume<\/strong><\/td>\n<td width=\"79\">50 \u03bcl<\/td>\n<td width=\"162\">\u2013<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n\t\t\t<\/div>\n    <\/div>\n","protected":false},"excerpt":{"rendered":"<p>Ilya GelGreen Ilya Taq Master Mix GelGree is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels.&nbsp;It is far more sensitive than SYBR\u00ae Safe. Unlike SYBR\u00ae dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally. GelGreen\u2122 &#8230; <a title=\"products\" class=\"read-more\" href=\"http:\/\/ilyabiotech.com\/eng\/products\/\" aria-label=\"Read more about products\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-11","page","type-page","status-publish"],"_links":{"self":[{"href":"http:\/\/ilyabiotech.com\/eng\/wp-json\/wp\/v2\/pages\/11"}],"collection":[{"href":"http:\/\/ilyabiotech.com\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/ilyabiotech.com\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/ilyabiotech.com\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/ilyabiotech.com\/eng\/wp-json\/wp\/v2\/comments?post=11"}],"version-history":[{"count":11,"href":"http:\/\/ilyabiotech.com\/eng\/wp-json\/wp\/v2\/pages\/11\/revisions"}],"predecessor-version":[{"id":2062,"href":"http:\/\/ilyabiotech.com\/eng\/wp-json\/wp\/v2\/pages\/11\/revisions\/2062"}],"wp:attachment":[{"href":"http:\/\/ilyabiotech.com\/eng\/wp-json\/wp\/v2\/media?parent=11"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}